PTPRF Knockout cell line (U-251 MG)

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PTPRF Knockout cell line (U-251 MG) Order

PTPRF Knockout cell line (U-251 MG)
Catalog#
YKO-H194
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PTPRF Knockout cell line (U-251 MG)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
PTPRF Knockout cell line (U-251 MG)
Species
Human
Gene Name
PTPRF
Gene ID
5792
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Accession
CVCL_E1E4(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 9 (based on transcript PTPRF-201)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PTPRF gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the U-251 MG cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PTPRF gene knockout PTPRF Knockout cell line (U-251 MG) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PTPRF Knockout cell line (U-251 MG)
PTPRF Knockout cell line (U-251 MG)
Catalog#
YKO-H194
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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